Part I: Drosophila Protocols

1. Immunostaining of the Developing Embryonic and Larval Drosophila Brain

Danielle C. Diaper and Frank Hirth

1. Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

David Jussen and Rolf Urbach

1. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling

Karoline F. Kraft and Rolf Urbach

1. Flybow to Dissect Circuit Assembly in the Drosophila Brain

Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker

1. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe

Benjamin Perruchoud and Boris Egger

1. Using MARCM to Study Drosophila Brain Development

Gudrun Viktorin

Part II: Other Arthropods

1. Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria

George Boyan and Yu Liu

Part III: Molluscs

1. Methods in Brain Development of Molluscs

Andreas Wanninger and Tim Wollesen

Part IV: Xenopus Protocols

1. In Situ Hybridization and Immunostaining of Xenopus Brain

Kai-li Liu, Xiu-mei Wang, Zi-long Li, and Ying Liu

1. Microinjection Manipulations in the Elucidation of Xenopus Brain Development

Cristine Smoczer, Lara Hooker, Saqib S. Sachani, and Michael J. Crawford

1. Morpholino Studies in Xenopus Brain Development

Jennifer E. Bestman and Hollis T. Cline

Part V: Zebrafish Protocols

1. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification

Gilbert Lauter, Iris Söll, and Giselbert Hauptmann

1. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish

Adèle Faucherre and Hernán López-Schier

1. Anatomical Dissection of Zebrafish Brain Development

Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins

Part VI: Chicken Protocols

1. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain

Richard P. Tucker and Qizhi Gong

1. Transplantation of Neural Tissue: Quail-Chick Chimeras

Andrea Streit and Claudio D. Stern

1. RNAi-Based Gene Silencing in Chicken Brain Development

Irwin Andermatt and Esther T. Stoeckli

Part VII: Mouse Protocols

1. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development

Jinling Liu and Aimin Liu

1. In Utero Electroporation to Study Mouse Brain Development

Emilie Pacary and François Guillemot

1. The Cre/Lox System to Assess the Development of the Mouse Brain

Claudius F. Kratochwil and Filippo M. Rijli